Basing on this principle, the cultural assemblages found in different layers can be assigned a chronological personality. In practice it is found that the mounds are disturbed9 by all sorts of pits and dumps.
For example, if the cultural contents of the lower deposit are Mauryan in character, appropriately this deposit may be assigned a date between 400-200 B. Similarly, if the cultural equipment of the upper deposit are of the Sunga period, this deposit has to placed between 200-73 B. Quite often, the archaeologist decided the change of stratum on the basis of the feed of the deposit.
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Total organic carbon and/or dinosaur bone bio-apatite was then extracted and pretreated to remove potential contaminants and concordant radiocarbon dates were obtained, all of which were similar to radiocarbon dates for megafauna. Walter Libby's team of collagen from "dense mid-shaft femur bones" of twelve extinct saber tooth tigers, [Smilodon] from the Le Brea Tar Pits of Los Angeles CA.
Key Words: Radiocarbon dating, dinosaur, bone collagen, organic carbon, bone bio-apatite, fossil wood, amber, megafauna Introduction Bone collagen and soft tissue were recently reported as having been discovered in a Tyrannosaurus dinosaur femur bone as well as other fossil bones from the Cretaceous period of the geologic column by Mary H. The RC ages for extracted bone collagen for Smilodon femurs ranged from 12,650 ±160 to 28,000 ±1400 RC years BP (Before the Present). Libby, the inventor of the radiocarbon dating method, "There is no known natural mechanism by which collagen may be altered to yield a false age." It is common practice to determine the age of bones by radiocarbon (RC) dating of extracted bone collagen but not of dinosaurs because they are assumed to have become extinct 65 million years BP and, therefore, too old for RC dating.
Radiocarbon dating may only be used on organic materials.
Typically (6): The above list is not exhaustive; most organic material is suitable so long as it is of sufficient age and has not mineralised - dinosaur bones are out as they no longer have any carbon left.